This study investigated the impact of freight rates on the transportation of farm produce through road in certain states located in the southwestern region of Nigeria. The study utilized a cross[1]sectional design. In order to collect data for the study, a survey consisting of 400 questionnaires was distributed among transporters and market sellers in the major markets of Ondo, Osun, and Ekiti states within the study area. Data presentation in this study involved the utilization of tables and charts, while the evaluation of the relationship amongst traffic congestion and agricultural product distribution features was conducted by regression analysis. The study's findings indicate that the supply of farm produce in the study area is adversely affected by fuel scarcity, high levels of extortion rates by customs, and long travel distances, which in turn lead to high freight rates. The research findings revealed a statistically significant correlation (r=0.705; p<0.05) between freight rates and the transportation of agricultural products. The research findings indicate that the delivery and distribution of farm produce in the study area are impacted by factors such as fuel scarcity, high extortion rates, and distance, which in turn affect freight rates. The study proposes, among other recommendations, the establishment of an agricultural output databank and storage facility in order to implement an environmentally conscious and efficient system to facilitate agricultural freight transportation in the study region.